Store output of matchPWM()
2
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mat149 ▴ 80
@mat149-11450
Last seen 20 days ago
United States

I'm having trouble finding a solution regarding the storage of output from matchPWM(). I'd like to store the output in xlsx/csv/txt file format. Trying to convert to dataframe after unlist() returns the error 'unimplemented type 'list' in 'encodeelement'

pwm<-PWM(adc,type="prob",prior.params = c(A=0.25,C=0.25,G=0.25,T=0.25))
pwm
          -4          -3           -2         -1           BP         +1
A 0.05377283 0.005631321 -0.017774842 0.01002784  0.326073962 0.04033945
C 0.04510563 0.115371305  0.022894940 0.11125792 -0.021888223 0.09707738
G 0.02962736 0.016846775 -0.006567253 0.03466881 -0.058405298 0.03089362
T 0.11013323 0.100789643  0.240086200 0.08268447 -0.007141397 0.07032860



i<-makeGRangesFromDataFrame(intsd)
mcols(i)[1]<-intsd$GID
mcols(i)[2]<-DNAStringSet(intsd$FASTA)
ir<-resize(i,width=29,fix="end")
irs<-getSeq(genome,ir)

irs
DNAStringSet object of length 20734:
        width seq                                           names               
    [1]    29 TGGACGTTCCTGACTGTCACGATAGATAG                 VEintron1
    [2]    29 ACTTGGCCATGTGCTAACAACTTCCATAG                 VEintron2
    [3]    29 TTTCTTTACTTCCTAATTTGTCTTAATAG                 VEintron3
    [4]    29 AGCGTTACTAATGAATTGTTTTTCCACAG                 VEintron4
    [5]    29 TGATTGTGATGGAACTGATGGCAGAACAG                 VEintron5
    ...   ... ...
[20730]    29 TGAGCAGTATTGAAGGCTGATCATTGCAG                 VEintron20730
[20731]    29 TGGAAGCGTCTAGAGCTAATCTCCCTTAG                 VEintron20731
[20732]    29 TGGAAGAGTTTAAAGCTAATTTGCCTTAG                 VEintron20732
[20733]    29 ATCCCTCCGTACATTTGACTGTTAGAAAG                 VEintron20733
[20734]    29 CGGCCGAAAACTCACAAACACCTGTTCAG                 VEintron20734


hitlist<-lapply(irs, FUN = matchPWM, min.score="90%", with.score=TRUE, pwm = pwm)
hitlist
$VEintron20730
Views on a 29-letter DNAString subject
subject: TGAGCAGTATTGAAGGCTGATCATTGCAG
views: NONE

$VEintron20731
Views on a 29-letter DNAString subject
subject: TGGAAGCGTCTAGAGCTAATCTCCCTTAG
views: NONE

$VEintron20732
Views on a 29-letter DNAString subject
subject: TGGAAGAGTTTAAAGCTAATTTGCCTTAG
views: NONE

$VEintron20733
Views on a 29-letter DNAString subject
subject: ATCCCTCCGTACATTTGACTGTTAGAAAG
views:
      start end width
  [1]    14  19     6 [TTTGAC]

$VEintron20734
Views on a 29-letter DNAString subject
subject: CGGCCGAAAACTCACAAACACCTGTTCAG
views:
      start end width
  [1]    10  15     6 [ACTCAC]


adf<-as.data.frame(do.call(cbind, hitlist)) 
Warning message:
In cbind(...) :
  number of rows of result is not a multiple of vector length (arg 14)

  VEintron20712 VEintron20713 VEintron20714 VEintron20722 VEintron20729
1        GCTCAC        TTTCAC        TTTCAC        TCTGAC        TTTCAA
2        GCTCAC        TTTCAC        TTTCAC        TCTGAC        TTTCAA
3        GCTCAC        TTTCAC        TTTCAC        TCTGAC        TTTCAA
4        GCTCAC        TTTCAC        TTTCAC        TCTGAC        TTTCAA
5        GCTCAC        TTTCAC        TTTCAC        TCTGAC        TTTCAA


write.table(adf,file="adf.txt")
Error in write.table(adf, file = "adf.txt") : 
  unimplemented type 'list' in 'EncodeElement'
biostrings Biostrings • 569 views
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1
Entering edit mode
mat149 ▴ 80
@mat149-11450
Last seen 20 days ago
United States

hitlist<-lapply(irs, FUN = matchPWM, min.score="90%", with.score=TRUE, pwm = pwm)
ot <- endoapply(hitlist, function(x) as(x, "IRanges"))
to<-SplitDataFrameList(ot)

SplitDataFrameList of length 20734

$VEintron1 DataFrame with 1 row and 1 column X <IRanges> 1 10-14

$VEintron2 DataFrame with 0 rows and 1 column

$VEintron3 DataFrame with 2 rows and 1 column X <IRanges> 1 5-9 2 22-26

...

<20731 more elements>
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1
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@james-w-macdonald-5106
Last seen 3 minutes ago
United States

Something like this?

ind <- as.logical(sapply(hitlist, length))
out <- do.call(c, lapply(hitlist, function(x) as(x, "IRanges")))
mcols(out)$seq <- do.call(c, lapply(hitlist, as.character))
mcols(out)$name <- names(hitlist)[ind]
write.table(as(out, "data.frame"), <name goes here>)
0
Entering edit mode

out <- do.call(c, lapply(hitlist, function(x) as(x, "IRanges")))

Error in do.call(c, lapply(hitlist, function(x) as(x, "IRanges"))) : 'what' must be a function or character string

do.call does not like 'c'/concatenate for the 'what' argument. any other suggestions?

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1
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Here's a self contained reproducible example, using part of the example in ?matchPWM

> library(Biostrings)
> data(HNF4alpha)
> library(BSgenome.Dmelanogaster.UCSC.dm3)
> chr3R <- Dmelanogaster$chr3R
> pfm <- consensusMatrix(HNF4alpha)
> pwm <- PWM(pfm)  

> fakeirs <- DNAStringSet(chr3R, seq(1, 5e5, 1000), width = 1000)
> hitlst <- lapply(fakeirs, matchPWM, min.score = "90%", pwm = pwm)
> out <- do.call(c, lapply(hitlst, function(x) as(x, "IRanges")))
> out
IRanges object with 13 ranges and 0 metadata columns:
           start       end     width
       <integer> <integer> <integer>
   [1]       767       779        13
   [2]       603       615        13
   [3]       429       441        13
   [4]       515       527        13
   [5]       141       153        13
   ...       ...       ...       ...
   [9]       226       238        13
  [10]       549       561        13
  [11]       481       493        13
  [12]       502       514        13
  [13]       750       762        13
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