Downloading Metagenomic Data with Higher Taxonomic Units
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ua51601 • 0
@223b975e
Last seen 24 days ago
Slovenia

Hello,

I am currently downloading relative abundance datasets using the script below. While the download completes successfully, all the features in the output are at the species level. However, I am also interested in obtaining relative abundances for higher taxonomic levels, such as genera and families.

In my previous work with MetaPhlAn outputs, the results typically included higher taxonomic units, but in this case, I am only receiving species-level data.

Does anyone know why this might be happening, or how I can modify the process to include higher taxonomic levels?

This is the format in which I would prefer to have the taxonomic units:

k__Bacteria k__Bacteria|p__Actinobacteria k__Bacteria|p__Actinobacteria|c__Actinobacteria k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales|f__Actinomycetaceae k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales|f__Actinomycetaceae|g__Actinobaculum k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales|f__Actinomycetaceae|g__Actinobaculum|s__Actinobaculum_massiliense

relative_abundance_data <- curatedMetagenomicData(".relative_abundance", dryrun = FALSE)
View(relative_abundance_data)

saveRDS(relative_abundance_data, file = "relative_abundance_data.rds")
print("ABUNDANCE DATA SAVED")
curatedMetagenomicData • 150 views
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Hi! I'm facing the same problem.

Is there any easy way to output relative abundances of higher taxonomic units (up to kingdom), similar to the output of MetaPhlAn? I hope you can resolve our doubt.

Thanks in advance.

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Entering edit mode
Levi Waldron ★ 1.1k
@levi-waldron-3429
Last seen 4 hours ago
CUNY Graduate School of Public Health a…

I'm sorry I missed the first post! Yes, it is easy to agglomerate ranks in curatedMetagenomicData datasets. See the example in the vignette section Agglomerate Ranks - you just use the mia::splitByRanks() function.

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