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Hello,
Total newbie here. Trying to analyze RNA-seq dataset with RSEM counts from TCGA.
First I need to QC and normalize the dataset. For some reason, rows are patients and columns are genes...confusing. Do I need to transpose first before using something like DESeq2?
Thank you.
Thank you.
If I have questions about QC/normalization, do I ask them here or ask a new question?
You can ask here, but please read the
DESeq2vignette first, which should answer most questions.OK thank you.