compute_fasta_digest does not match Salmon index hash
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Alex • 0
@14d9d0d2
Last seen 4 days ago
United States

Note sure where else to ask this, but I have been using tximport for importing Salmon quantifications against custom transcriptomes into R.

I don't always use salmon mapping mode for quantification, so by default I run compute_fasta_digest as was recommended to get the txomes Seq and Name hashes for when I generate a TxDb, but upon further inspection this does not seem to always work. Either the SeqHash or the NameHash from FastaDigest seems different than the salmon index, meaning my prebuilt linkedTxome and TxDb don't always work.

Does anybody know how to reliably compute the salmon hash values without fully indexing?

Salmon v1.10.0
fasta_digest v0.1.2
tximeta v1.16.0
R v4.2.3

tximeta • 31 views
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