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How can I develop an MSstat workflow (codes) to analyze DIA data obtained after APEX proximity labeling of the Kappa opioid receptor at different time points upon ligand stimulation?
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Are there any other explanatory factors or covariates in your experiment other than time point? Do you have biological replicates at each time point?
I think it would help the MSstats authors for you to describe your experiment more completely, especially the number of samples. No one will be able to provide you with code I think without knowing the experimental design and the software you are using for quantification.