Hi, I was trying to trying counts reads mapped onto a bed file,
I checked the workflow of "DESeq2" ,
gtffile <- ("mm9genes.gtf")
library("GenomicFeatures")
(txdb <- makeTxDbFromGFF(gtffile, format="gtf", circ_seqs=character()))
(ebg <- exonsBy(txdb, by="gene"))
library("GenomicAlignments")
library("BiocParallel")
register(MulticoreParam())
se <- summarizeOverlaps(features=ebg, reads=bamfiles,
mode="Union",
singleEnd=TRUE,
ignore.strand=TRUE,
fragments=FALSE )
I am not sure I understood the code completely, but my understanding is that they extract the exons locations grouped by genes (by using GenomicFeatures packages), and do the counting.
but what if I want to count the reads overlap with genomic features described in a bed file or gtf converted from the bed file? the counterpart of "ebg" in the example.
