Entering edit mode
The warning in creating TxDb:
gtffile <- file.path("PATH/Mus_musculus.GRCm38.86.gtf") txdb <- makeTxDbFromGFF(gtffile, format="gtf") Import genomic features from the file as a GRanges object ... OK Prepare the 'metadata' data frame ... OK Make the TxDb object ... OK Warning messages: 1: Named parameters not used in query: internal_chrom_id, chrom, length, is_circular 2: Named parameters not used in query: internal_id, name, type, chrom, strand, start, end 3: Named parameters not used in query: internal_id, name, chrom, strand, start, end 4: Named parameters not used in query: internal_id, name, chrom, strand, start, end 5: Named parameters not used in query: internal_tx_id, exon_rank, internal_exon_id, internal_cds_id 6: Named parameters not used in query: gene_id, internal_tx_id
It doesn't change if I add the other arguments as "dbxrefTag= "GeneName" " or "circ_seqs=character()"
of course I insert the correct path, so PATH is the full path where the gtf file exists: