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@adaikalavan-ramasamy-675
Last seen 10.0 years ago
Sorry if this question is documented somewhere but I could not find the answer to it. Feel free to point this out to me ? I am trying to get some gene ontology info from the chip Hu35KsubA for which I think I need the package "hu35ksuba". Note that this is not the same as "hu35ksubacdf" which is already provided by BioConductor. 1) Am I correct in thinking that I need "hu35ksuba" only ? 2) I do not think someone has built this for R. Can I download the CDF file and then use make.cdf.env() to do this ? If anyone has this and is willing to share, please send them to me offline and I would be very grateful. 3) Where do I get the CDF file from ? I cannot see it on this site http://www.affymetrix.com/support/technical/byproduct.affx?product=hu3 5k Thank you. Regards, Adai
hu35ksuba cdf hu35ksuba cdf • 814 views
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@james-w-macdonald-5106
Last seen 30 minutes ago
United States
Adaikalavan Ramasamy wrote: > Sorry if this question is documented somewhere but I could not find the > answer to it. Feel free to point this out to me ? > > I am trying to get some gene ontology info from the chip Hu35KsubA for > which I think I need the package "hu35ksuba". Note that this is not the > same as "hu35ksubacdf" which is already provided by BioConductor. > > 1) Am I correct in thinking that I need "hu35ksuba" only ? > > 2) I do not think someone has built this for R. Can I download the CDF > file and then use make.cdf.env() to do this ? If anyone has this and is > willing to share, please send them to me offline and I would be very > grateful. You are confused; make.cdf.env() makes the environment for the hu35ksubacdf package, not the annotation package. > > 3) Where do I get the CDF file from ? I cannot see it on this site > http://www.affymetrix.com/support/technical/byproduct.affx?product=h u35k As noted, you don't need the CDF file (which simply maps chip coordinates to probes). You will need to use the ABPkgBuilder() function in AnnBuilder. For this you will need a text file containing a column of Affy IDs and a column of Entrez Gene IDs, which you can get from this file: http://www.affymetrix.com/Auth/analysis/downloads/taf/Hu35KsubA_annot_ csv.zip I think the vignette for this package is relatively straightforward. See ABPrimer for more info. Best, Jim > Thank you. > > Regards, Adai > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor -- James W. MacDonald Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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Yes, you are right. I was confusing building meta packages with building CDFs. Thank you for the explanation. Also thanks to Jeff Gentry (and indirectly to Jianhua Zhang) for providing me the R package in an offline mail saving me the trouble of trying to build it from scratch. Regards, Adai On Tue, 2005-06-21 at 09:09 -0400, James W. MacDonald wrote: > Adaikalavan Ramasamy wrote: > > Sorry if this question is documented somewhere but I could not find the > > answer to it. Feel free to point this out to me ? > > > > I am trying to get some gene ontology info from the chip Hu35KsubA for > > which I think I need the package "hu35ksuba". Note that this is not the > > same as "hu35ksubacdf" which is already provided by BioConductor. > > > > 1) Am I correct in thinking that I need "hu35ksuba" only ? > > > > 2) I do not think someone has built this for R. Can I download the CDF > > file and then use make.cdf.env() to do this ? If anyone has this and is > > willing to share, please send them to me offline and I would be very > > grateful. > > You are confused; make.cdf.env() makes the environment for the > hu35ksubacdf package, not the annotation package. > > > > > 3) Where do I get the CDF file from ? I cannot see it on this site > > http://www.affymetrix.com/support/technical/byproduct.affx?product =hu35k > > As noted, you don't need the CDF file (which simply maps chip > coordinates to probes). > > You will need to use the ABPkgBuilder() function in AnnBuilder. For this > you will need a text file containing a column of Affy IDs and a column > of Entrez Gene IDs, which you can get from this file: > > http://www.affymetrix.com/Auth/analysis/downloads/taf/Hu35KsubA_anno t_csv.zip > > I think the vignette for this package is relatively straightforward. See > ABPrimer for more info. > > Best, > > Jim > > > > > Thank you. > > > > Regards, Adai > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > >
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