You could do this using LME. It will be slow. --Naomi At 05:47 AM 6/2/2005, HAG wrote: >Dear list, > >I have an experiment with 24 arrays. >I have 3 factors each with 2 levels and 12 blocks (1,1,2,2,3,3,....,12,12). >Each gene is replicated 4 times on each array. > >I know how to fit blocks as random as explained in the Limma manual >(Technical replication I) >I know how to account for the replication within arrays. > > >Is it possible to fit random block effect and technical replicates within >array at the same in linear model using Limma ? > >Thanks >Peter > >_______________________________________________ >Bioconductor mailing list >Bioconductor at stat.math.ethz.ch >https://stat.ethz.ch/mailman/listinfo/bioconductor Naomi S. Altman 814-865-3791 (voice) Associate Professor Bioinformatics Consulting Center Dept. of Statistics 814-863-7114 (fax) Penn State University 814-865-1348 (Statistics) University Park, PA 16802-2111