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yangwanqi
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@6e5d153a
Last seen 4.1 years ago
# tppData <- readRDS("../data/tppData.Rds")
sessionInfo( )
Please help us to know how to convert the TPP result to a "tidy format".
A first comment upfront: It would have been hard for me to understand your question without the email you've sent before. To clarify: the problem is concerning the conversion of the
ExpressionSet
structure obtained after data import of a "Thermal proteome profiling" experiment using theTPP
package and then converting this to a long format data frame which theNPARC
package needs as input. (https://bioconductor.org/packages/release/bioc/vignettes/NPARC/inst/doc/NPARC.html)Secondly, it would help if you also post the out put appearing in your console upon typing
sessionInfo().
Assuming you have imported data using the functions from the
TPP
package similar to:you can convert the obtained
ExpressionSet
into a long format data frame by using the Bioconductor packagebiobroom
:This results in the following data frame:
To adapt the column names to match the ones from the
NPARC
example:you can now use
dplyr
:Based on this data frame you should be able to perform the
NPARC
analysis as described in the vignette.