User: lestelles

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lestelles0
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Posts by lestelles

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Comment: C: Question about contrast matrix using limma
... Thanks, I wanted to be sure there is no other way to do it. I understand I need to select genes with a wide confidence interval and a small logFC to find non-DE genes between A1 and B2. ...
written 3.6 years ago by lestelles0
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Comment: C: Question about contrast matrix using limma
... Thank you for your answer. I should probably have mentioned that I have used limma for factorial designs before for other experiments. I am able to find the differentially expressed transcripts between conditions. But in my case I am interested in the intersection of transcripts which are DE in (A1- ...
written 3.6 years ago by lestelles0 • updated 3.6 years ago by Gordon Smyth39k
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Question about contrast matrix using limma
... Hi, I am analysing RNA-seq data of two genotypes (A and B) in order to detect the transcripts that are involved in a physiologic change that takes place earlier in genotype A and later in genotype B. For genotype A there are 2 time points (A0 and A1) and for genotype B there are 3 time points (B0, ...
limma rna-seq limma contrast matrix written 3.6 years ago by lestelles0 • updated 3.6 years ago by Gordon Smyth39k

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