User: xpeng

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xpeng60
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Last seen:
13 years, 9 months ago
Joined:
14 years, 6 months ago
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x****@utk.edu

Posts by xpeng

<prev • 6 results • page 1 of 1 • next >
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Answer: A: presence/absence call using oligo array?
... Thanks a lot. What are the arrays or other technologies to survey the presence/absence of genes in a large scale? I noticed that Winzeler's lab published a paper using a Affy-like custom-made, high-density oligo array (Le Roch et al, Science, 301:1503-1508). Best, Xinxia >===== Original Mess ...
written 13.8 years ago by xpeng60
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Answer: A: presence/absence call using oligo array?
... I agree. Thanks a lot. Even I want to find differentially expressed genes. How do I normalize? The oligos are designed based on parasite genes, while samples from uninfected tissue have no parasite RNAs. Best, Xinxia >===== Original Message From Sean Davis ===== >On 1/13/06 3:07 AM, "xpeng ...
written 13.8 years ago by xpeng60
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Answer: A: presence/absence call using oligo array?
... Hi Amy, Thanks for the reply. You are right. The uninfected tissue is a blank, in theory. But the real problem is that some probes cross-hybridize with house RNAs. I do not know the oligo sequence yet. They know that there are more host RNAs than parasite RNAs in the sample from the infected tissu ...
written 13.8 years ago by xpeng60
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presence/absence call using oligo array?
... Dear Bioc, Has someone ever used long oligo arrays to test if genes were expressed or not? I have two microarray slides on which oligonucleotides (65mer) for genes from a parasite genome were spotted. One gene has one oligo but spotted twice on each array. One slide has a RNA sample extracted from ...
microarray oligo written 13.8 years ago by xpeng60
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Answer: A: Cho et al.'s Expression Dataset in Yeast
... Hi, The following website provides a combined data file in tab-delimited format: http://genome-www.stanford.edu/cellcycle/data/rawdata/ Best, Xinxia >===== Original Message From Ho-Joon Lee ===== >Dear All, > >I would like to ask about the following yeast cell cycle dataset, > &g ...
written 14.5 years ago by xpeng60
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Answer: A: Pearson correlation and p-values for a matrix
... Hi Dren, I had the similar question, then I wrote my own C program to calculate correlation matrices (2-3min for a 8000 x 60 matrix). You can contact with me directly (xpeng@utk.edu), it need some extra works to get p-values. I will post it somewhere online if there are enough interests. Best, Xin ...
written 14.5 years ago by xpeng60

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