User: Seymoo

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Seymoo0
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Posts by Seymoo

<prev • 27 results • page 1 of 3 • next >
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intra-cluster silhouette width
... I am wondering how to make a silhouette plot on the results from Mclust packge, where measure of silhouette for each sample in each group is shown? Using "Fcp" package I can check the average silhouette. mb = Mclust(iris[,-5], 3) cs = cluster.stats(dist(iris[1:4]), mb$classification) #instal ...
mclust silhouette fcp written 7 weeks ago by Seymoo0
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Comment: C: limma contrast matrix
... Hi @Aaron Lun . I didnt want to post a new question because I am actually a bit confused with the reversing that you just mentioned above ( e.g., N50_C - N50_D instead of N50_D - N50_C ), because by doing so this can swap the number of detected DE. I am wondering if what is generally accepted when I ...
written 7 weeks ago by Seymoo0
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Comment: C: convert Gene collection to gene list for camera function
... Thanks James! ...
written 8 weeks ago by Seymoo0
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convert Gene collection to gene list for camera function
... I want to use `camera` function in limma. I downloaded xml file from MSigDB and loaded it but I am wondering how can I make it to a compatible list for camera function as it has been mentioned in here that GEneCollection need to be converted to a list. what I have done is gsc <- getBroadSets(" ...
limma gseabase written 9 weeks ago by Seymoo0 • updated 9 weeks ago by James W. MacDonald46k
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GSA for comparison of geneset enricment analysis between multiple class
... I have a gene expression data `AllExp` and a data.frame `info` with sample name of `AllExp` as row names and first column in it specifies groups that each sample belongs to. I am trying to perform a a gene set enrichment analysis using GSA package. but I get following error `Error in cut.default(s ...
gsa written 9 weeks ago by Seymoo0
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Answer: A: Changing SVM-Kernal in geNetClassifier function
... @saibar Hi Sara, Thanks for making this great package. I am wondering if you add cost argument in the formula to be set by users or if you could explain how to modify it the way it is right now ? I am using Caret package to find optimal cost to be used in the function and I would like to adjust co ...
written 4 months ago by Seymoo0
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Comment: C: Multiple comparison of coefficients in limma
... Thanks a lot for explanation @Aaron Lun. ...
written 4 months ago by Seymoo0
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Comment: C: Multiple comparison of coefficients in limma
... Hi James, So because now I am comparing them against the mean, to do similar comparison would it be better to formulate it like this cm1 <- makeContrasts(G1VsG2 = G1-G2, G1VsG3 = G1-G3, G1VsG4 = G1-G4, G2VsG3 = G2-G3, ...
written 4 months ago by Seymoo0
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Multiple comparison of coefficients in limma
... In a cohort of cancer samples there are 4 groups, G1 to G4. I want to perform differential gene expression between group1 Vs all other groups, i,e, G1 = G1-(G2+G3+G4)/3, to determine DEGs for group 1. I could just find an example in limma guide where pair-wise comparisons being made ( under 9.3. se ...
limma written 4 months ago by Seymoo0 • updated 4 months ago by James W. MacDonald46k
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Comment: C: Probe summerization of Human HT-12 V4 BeadChip arrays
... Hi @Mark, I am using BioC_mirror: https://bioconductor.org Using Bioconductor 3.4 (BiocInstaller 1.24.0), R 3.3.2 (2016-10-31) I have not manage to solve the problem yet. I manage to download the data matrix with gse <- getGEO("GSE73255", GSEMatrix = FALSE) but eset <- as(gse, "Expres ...
written 4 months ago by Seymoo0

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