User: inah

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inah0
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Posts by inah

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read count summed over exons is greater tham the gene-level read count using featureCounts
... Hi,   I have been using featureCounts to obtain both exon- and gene-level read counts (reads were aligned with STAR). For one particular gene (ARID5B, which has 12 exons, 5 unique to one isoform, 2 unique to another isoform and 5 shared), I find that the read count summed over the 12 exons is greate ...
rsubread featurecounts exon mrnaseq written 5 months ago by inah0 • updated 5 months ago by Wei Shi2.7k
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exon counting with featureCounts
... Hi,   I want to obtain read counts at the exon level using featureCounts. I run Rsubread and use these options: annot.ext="/home/inah/RefGTF/GRCh38/annotation/Homo_sapiens.GRCh38.85.gtf", isGTFAnnotationFile=TRUE, GTF.featureType="exon",  GTF.attrType="exon_id",  useMetaFeatures=FALSE The resulti ...
rnaseq written 5 months ago by inah0

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