User: justinyi10

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justinyi1010
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Posts by justinyi10

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Comment: C: How to find Geometric Mean from gated Quantibeads
... After using the customized function above on my quantibead sample, I get the warning message below. Why would this be? Also, the geometric means seem to be way out of the expected range (comparing against the flow cytometer software). Does the function above return the geometric mean of the number o ...
written 2.1 years ago by justinyi1010
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How to construct histogram of Quantibead Statistics
... Is there a way to construct a histogram (with FL2 on the x axis and counts on the y axis) to get statistics on quantibeads which have been run through the flow cytometer? I am looking to get the FL2 geometric means of the 4 quantibeads  ...
bioconductor written 2.1 years ago by justinyi1010
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Comment: C: How to find Geometric Mean from gated Quantibeads
... How would you extract the fluorescence intensity matrix? ...
written 2.1 years ago by justinyi1010
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How to find Geometric Mean from gated Quantibeads
... Is there a way to find the geometric means of different fluorescence intensities that arise when gating quantibeads in Bioconductor? ...
flowworkspace opencyto written 2.1 years ago by justinyi1010 • updated 2.1 years ago by Jiang, Mike1.2k
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Trouble Installing cytoUtils
... Hello, I am trying to install cytoUtils using the devtools :: install_github("RGLab/cytoUtils") command. I receive an error that reads the following: Error: package or namespace load failed for ‘cytoUtils’ in dyn.load(file, DLLpath = DLLpath, ...): unable to load shared object '/Library/Frameworks ...
cytoutils written 2.2 years ago by justinyi1010
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Error during RProtoBufLib Installation
... I am trying to install RProtoBufLib from github, however I receive the error below. I have downloaded the GNU Automake zip but I don't know what to do next. Help would be appreciated 'aclocal-1.14' is missing on your system.          You should only need it if you modified 'acinclude.m4' or         ...
software error written 2.2 years ago by justinyi1010
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Comment: C: Measure Median Fluorescence Intensity in openCyto
... I get a 404 Not Found error when using the command shown below. I notice that the url is (https://api.github.com/repos/RGLab/RProtoBufLib/zipball/master). Is there a way to change the url so that the branch is 'trunk' and not 'master' devtools::install_github("RGLab/RProtoBufLib") ...
written 2.2 years ago by justinyi1010
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Comment: C: Measure Median Fluorescence Intensity in openCyto
... cytoUtils is dependent on RProtoBufLib but RProtoBufLib is not available for R 3.4.1. Therefore I am unable to install cytoUtils, as I am hit with an error (see below). What should I do? ERROR: dependency ‘RProtoBufLib’ is not available for package ‘cytoUtils’ * removing ‘/Library/Frameworks/R.fram ...
written 2.2 years ago by justinyi1010
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Measure Median Fluorescence Intensity in openCyto
... I am using openCyto to gate CD markers, but instead of returning counts (using getPopStats function) I was wondering if there is a way to return the Median Fluorescence Intensity (MFI) for each sample in my gating set. ...
opencyto written 2.2 years ago by justinyi1010 • updated 2.2 years ago by Jiang, Mike1.2k
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How to construct a polygon gate?
... Hello, I am currently using the flowClust.2d and boundary gating methods to gate my samples, however I was wondering if it's possible to construct a polygon gate so that I can be more specific with the regions I capture. Thanks. ...
opencyto gating gating written 2.2 years ago by justinyi1010

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