User: susobhanbaidya

Reputation:
0
Status:
New User
Location:
Last seen:
2 months, 2 weeks ago
Joined:
3 months ago
Email:
s*************@gmail.com

Posts by susobhanbaidya

<prev • 19 results • page 1 of 2 • next >
0
votes
0
answers
282
views
0
answers
Off Target region
... I have  a question about the the off target region.  suppose the locus is 1000 for a target frame of 20bp in the sequence. Now what will be the region of consideration for the off target analysis. What i understood from papers and links, that  it should be plus/minus 500bp from target region at ...
crisprseek offtarget analysis written 12 weeks ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Comment: C: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... hanks Julie,  Can you please tell me about  the the off target region. I mean to say.  suppose the location is 1000 for a frame of 20bp in the sequence. Now what will be the region of consideration for the off target analysis. As far my knowledge it should be plus/minus 500bp at max. That means ...
written 12 weeks ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
...   Yes I got your attached file. Thank you Julie. I understood Score difference Zero means Common GRNA. Q1. But Score Difference One means what ? Q2. Still I want to know the formula of sgRNA efficiency score which is fractional.  How it is coming. Q3. High Score means what ? is it stable sgRN ...
written 12 weeks ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Thank you julie, Can you tell me, in the column of F in xls sheet sgrna efficiency means what? What is the scoring mechanism ( Doench Activity score or Efficiency score ) ...
written 12 weeks ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... I cant get your answer, if you do not mind, can you please write complete code for that  example,  I am very new with R. Keenly waiting for your reply ...
written 3 months ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Your reply was Q5: what will be the commands for only  potential off target analysis of a given  input sequence only. Not species specific  Can you please clarify? Do you mean you are not interested in the genome-wide off-target analysis?  Yes  I want to get all potential off-target GRNAs from ...
written 3 months ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Thanks Julie again, Your previous reply was   Susobhan, Bioconductor recently transitioned from SVN to github. I need to set up account to clone my package, do testing and make changes if needed. It will take me quite sometime maybe days if I do have a big chunk of time to do this. Therefore, I ...
written 3 months ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Thanks Julie for your enormous effort. I have 2/ 3 precise question    Qs1 > Can I compare more than 2 that is 3  sequences by any other command.   Qs2.  What is the meaning of Target in  sequence 1 one and Target in  sequence 2. if in same xls file , we are getting  common GRNAs , then why ...
written 3 months ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Comment: C: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Yes Please help me Julie.... Those sequences has the length of 1kb base pair  and it has 10%-20% variation of bp between them. Keenly waiting for your reply.   ...
written 3 months ago by susobhanbaidya0
0
votes
12
answers
478
views
12
answers
Answer: A: 'x' has "out of limits" views ,Issue with the file size!! Compareequence functio
... Madam I tried to follow your suggestion , but same problem *************** Should I give  small sequence for comparing two sequence  with only 1SNP. ***************** My input files were about 1Kb with 10-20 % variation (of nucleotide between those files).   > inputFile1Path <- DNAStringSe ...
written 3 months ago by susobhanbaidya0

Latest awards to susobhanbaidya

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.2.0
Traffic: 299 users visited in the last hour