User: yingyingguo0228

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Posts by yingyingguo0228

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Answer: A: Error in frameCounting using riboSeqR
... Hi Tom, I just tried again. It turns out that there is a blank space after each header of fastq file. So I should use command "sed 's/ cdna.*$//' Homo_sapiens.GRCh38.cdna.all.fa > humancdna.fa"​ instead of  "sed 's/cdna.*$//' Homo_sapiens.GRCh38.cdna.all.fa > humancdna.fa"​.  I did not rea ...
written 2.2 years ago by yingyingguo02280
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Answer: A: Error in frameCounting using riboSeqR
... Hi Tom, Thank you for your reply! At the begining, I found people having the same problems as me. They fixed their problem by making header name of their fastq file and bowtie output the same. so I checked mine and noticed that the header of my fastaq file is different from bowtie output.  So I us ...
written 2.2 years ago by yingyingguo02280
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Error in frameCounting using riboSeqR
... hi I am using riboSeqR to analyze ribosome profling data. However, after framecounting, all the frame counts are 0. Here is what I have done from the beginning. I removed the linker sequence and timmed the reads first, then align all the reads to ncRNA.fq and collect unaligned reads. Then I used b ...
R riboseqr riboseq written 2.2 years ago by yingyingguo02280

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