User: Samuel Ahuno

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Posts by Samuel Ahuno

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Answer: A: Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Many Thanks, Sam! I will look into the codes and get back to the community on that.   Best Samuel ...
written 10 months ago by Samuel Ahuno0
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Answer: A: Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Kindly find below the Comp Matrix from FlowJo. Any help will be highly appreciated. https://drive.google.com/open?id=1MIq65H71aXhx_hAr0G_i6YXlNY_-mZis ...
written 10 months ago by Samuel Ahuno0
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Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Dear all, I used the spillover() function in flowCore to generate a spillover matrix with a bunch of single-stained .fcs and an unstained sample. Strangely, I compared this spillover matrix from R with that of FlowJo Compensation Matrix and found that they were dissimilar. Perhaps, my approach wa ...
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Answer: A: How do you re-order .fcs files in a flowSet?
... ...Oh I missed that! Thanks, Jaro!   Best, Sam ...
written 10 months ago by Samuel Ahuno0
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How do you re-order .fcs files in a flowSet?
... Hi, I have a series of single-stained compensation controls (fcs files) loaded into  flowSet object which I would want to re-order to be used for calculating my spillover.   Best Sam   ...
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Answer: A: Windows 10 openCyto Install: installation path not writeable, unable to update p
... Hi there, Sometimes I could be because you have multiple R programs opened for example having "R studio" and "R base" running at the same time. Close one of these programs and lets see whether is resolved. Best Sam ...
written 11 months ago by Samuel Ahuno0
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Answer: A: spillover::flowCore problem: Error in match(unstained, sampleNames(x))
... Hi Daniel, I am not sure what you did exactly for your codes to work. I have tried using the "regexpr" and changing statics to "mode" and "max" but then I get the same error message; Error in match(unstained, sampleNames(x)) : 'match' requires vector arguments   Best Sam ...
written 11 months ago by Samuel Ahuno0
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spillover::flowCore problem: Error: Unable to match stains with controls based on intensity: a single stain matches to several multiple controls.
... My spillover matrix fails to generate. Details of problem below. Any help in that regard will be gladly appreciated. Thanks ​ myflowset3 = read.flowSet(files=list.files(path=".",pattern="fcs", full.names=TRUE)) summary(myflowset3) spx=spillover(myflowset3 , unstained = "Sample 2_Unstained.fcs", ...
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Answer: A: Windows 10 openCyto Install: installation path not writeable, unable to update p
... Hey my friend..... Try running R or R-studio as administrator. Simply right-click the "R" icon on your start and run as administrator if using windows. I hoe this helps! Best Sam   ...
written 15 months ago by Samuel Ahuno0

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