User: Samuel Ahuno

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Ghana/KNUST
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Posts by Samuel Ahuno

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Answer: A: Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Many Thanks, Sam! I will look into the codes and get back to the community on that.   Best Samuel ...
written 7 weeks ago by Samuel Ahuno0
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Answer: A: Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Kindly find below the Comp Matrix from FlowJo. Any help will be highly appreciated. https://drive.google.com/open?id=1MIq65H71aXhx_hAr0G_i6YXlNY_-mZis ...
written 7 weeks ago by Samuel Ahuno0
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Spillover matrix in FlowCore doesn't match Compensation Matrix from FlowJo
... Dear all, I used the spillover() function in flowCore to generate a spillover matrix with a bunch of single-stained .fcs and an unstained sample. Strangely, I compared this spillover matrix from R with that of FlowJo Compensation Matrix and found that they were dissimilar. Perhaps, my approach wa ...
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Answer: A: How do you re-order .fcs files in a flowSet?
... ...Oh I missed that! Thanks, Jaro!   Best, Sam ...
written 8 weeks ago by Samuel Ahuno0
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How do you re-order .fcs files in a flowSet?
... Hi, I have a series of single-stained compensation controls (fcs files) loaded into  flowSet object which I would want to re-order to be used for calculating my spillover.   Best Sam   ...
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Answer: A: Windows 10 openCyto Install: installation path not writeable, unable to update p
... Hi there, Sometimes I could be because you have multiple R programs opened for example having "R studio" and "R base" running at the same time. Close one of these programs and lets see whether is resolved. Best Sam ...
written 8 weeks ago by Samuel Ahuno0
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Answer: A: spillover::flowCore problem: Error in match(unstained, sampleNames(x))
... Hi Daniel, I am not sure what you did exactly for your codes to work. I have tried using the "regexpr" and changing statics to "mode" and "max" but then I get the same error message; Error in match(unstained, sampleNames(x)) : 'match' requires vector arguments   Best Sam ...
written 9 weeks ago by Samuel Ahuno0
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spillover::flowCore problem: Error: Unable to match stains with controls based on intensity: a single stain matches to several multiple controls.
... My spillover matrix fails to generate. Details of problem below. Any help in that regard will be gladly appreciated. Thanks ​ myflowset3 = read.flowSet(files=list.files(path=".",pattern="fcs", full.names=TRUE)) summary(myflowset3) spx=spillover(myflowset3 , unstained = "Sample 2_Unstained.fcs", ...
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Answer: A: Windows 10 openCyto Install: installation path not writeable, unable to update p
... Hey my friend..... Try running R or R-studio as administrator. Simply right-click the "R" icon on your start and run as administrator if using windows. I hoe this helps! Best Sam   ...
written 6 months ago by Samuel Ahuno0

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