User: emanuelvgoncalves

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Posts by emanuelvgoncalves

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Comment: C: Adaptation of DESeq/edgeR model for CRISPR drop-out screen
... Hi, Yes I'm familiar with that pipeline and I'm doing something similar to that but with DESeq2. The problem is that there are other potential artefacts of CRISPR data, e.g. copy-number, that need to be taken into account. A couple of methods account for this, one using a linear model with genes c ...
written 17 days ago by emanuelvgoncalves0
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Adaptation of DESeq/edgeR model for CRISPR drop-out screen
... Hi, Currently I use DESeq2 to analyse large scale CRIPSR loss-of-function screens (gene KO), where cell viability is measured with targeted sequencing, raw counts, for control and KO conditions. All great so far although CRISPR has a couple of other artefacts that need to be taken in consideration ...
edger deseq deseq2 crispr written 18 days ago by emanuelvgoncalves0 • updated 17 days ago by Aaron Lun17k

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