User: zoukai3412085

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Posts by zoukai3412085

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Reference annotation file contains only transcript and CDS information, how to analyze the different expression of genes?
... hello, everyone, My reference annotation gff file, which was downloaded from gigaDB(http://gigadb.org/dataset/100209), contains only transcript ID and CDS information like this: [root@xueduanliu Ginkgo_RNA_sequencing_analysis]# cat Ginkgo_biloba.gff | head -n 10 C24882126   Cufflinks    mRNA   3 ...
transcription hisat2 written 24 months ago by zoukai34120850
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Comment: C: How to use limma to do regularization for Ballgown
... Thank you so much! ...
written 24 months ago by zoukai34120850
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How to use limma to do regularization for Ballgown
... Hi, In the Nat Prot paper about Ballgown, it's said as follow: Note that Ballgown’s statistical test is a standard linear model-based comparison. For small sample sizes (n < 4 per group), it is often better to perform regularization. This can be done using the limma package in Bioconductor.   ...
limma rna-seq written 24 months ago by zoukai34120850 • updated 24 months ago by Aaron Lun25k

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