User: colore

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colore0
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Normalize depth before calculating TPM
... Hi We have RNA-seq data (three biological replicates in a single condition) and we want to calculate TPM with the sole purpose of ranking genes by expression level (in quantiles) . I understand that, while TPM uses the total reads number to normalize sequencing depth and library size, for DE  is t ...
rna-seq tpm library size factor written 24 months ago by colore0

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