User: uguy

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uguy0
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Posts by uguy

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Comment: C: DESeq2: normalization of experiments sequenced separately
... I understand your point.  But I then want to use a method that takes reads counts as input and I wonder if it's a problem that the sequencing depth is not normalized between the two datasets. (no comparison against the control reads counts). ...
written 9 weeks ago by uguy0
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Comment: A: DESeq2: normalization of experiments sequenced separately
... Thanks for answering me. There is two type of acclimated cells that were exposed to several stresses. The two types of acclimation were separated for sequencing step in two runs. The time points are not the same accross experiments and there is three biological replicates for each time point. And y ...
written 9 weeks ago by uguy0
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DESeq2: normalization of experiments sequenced separately
... Hi, I am analyzing RNAseq data of two experiments with multiple time points and their own control condition. These two experiments were sequenced in two separate runs. I wonder if I can merged the two raw counts tables and normalize them using the DESeq2 size factors method, or do I need to normali ...
rnaseq normalization deseq2 written 10 weeks ago by uguy0

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