User: davidjameswheatcroft

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Posts by davidjameswheatcroft

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Comment: C: WGCNA: intrepreting grey unassigned "module"?
... Ah, fantastic! Thanks for the help. The few DE genes is something that's been found a few times independently in these species, so we think it does represent the biology. Increasing deepSplit seems to have worked, producing three modules (between 80 to 160 genes) whose eigengenes are significantly ...
written 5 weeks ago by davidjameswheatcroft0
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Answer: A: WGCNA: intrepreting grey unassigned "module"?
... Thanks very much for your reply! It's fantastic that you respond to support questions... The two species are very closely related songbird species (< 1my since last common ancestor). Sequencing was done on a smallish brain region on 5 individuals from each species (and 3 hybrids). The counts wer ...
written 5 weeks ago by davidjameswheatcroft0
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WGCNA: intrepreting grey unassigned "module"?
... Hi all, I'm just getting into using WGCNA and had a question about my last run through. I'm looking at differences between two species in brain gene expression using RNAseq data. WGCNA produced 4 modules including the set of unassigned genes (labeled module 0 or the grey module). The grey module w ...
rnaseq wgcna gene expression co-expression network written 5 weeks ago by davidjameswheatcroft0 • updated 5 weeks ago by Peter Langfelder1.4k

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