User: k.teijgeler

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Posts by k.teijgeler

<prev • 16 results • page 1 of 2 • next >
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Comment: C: how to use the flowCore spillover function
... Thanks a lot for the thorough explanation and quick response Jake, and also for correcting my typo! spillover <- spillover(fcs , unstained = 17 , fsc ="FSC.A" , ssc ="SSC.A" , useNormFilt = TRUE, patt="FSC.A|SSC.A|BV|PE|Alexa|BUV|APC|FITC", method="mean") This one works for me :) ...
written 2 days ago by k.teijgeler0
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how to use the flowCore spillover function
... Hi, i have been trying to convert a gatingset to a flowJO workspace object by using the gatingset2FlowJO function from cytoML. After some experimentation i succeeded. However i noticed that only my transformation was in the flowJO workspace and not the compensation. The problem seems to be that i do ...
flowcore spillover gatingset2flowjo written 5 days ago by k.teijgeler0 • updated 5 days ago by Jake Wagner100
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Comment: C: polyFunctions gate in OpenCyto 1.20
... Hey Jiang, sorry for the delay i am just back from holiday. I just checked and it now works in 3_8. Thanks a lot for the quickfix :) ...
written 15 days ago by k.teijgeler0
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Comment: C: polyFunctions gate in OpenCyto 1.20
... I wish that was an easy option for me. I want a solution on an R shiny server i am using, so i am a 100% everyone uses the same packages and files ect. Using separate PC's has always been problematic, some people have more or less recent versions. Thats why i am currently working on a R shiny serve ...
written 28 days ago by k.teijgeler0
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Answer: A: polyFunctions gate in OpenCyto 1.20
... When using Bioconductor 3.7 with openCyto 1.20.2, it is problematic to have + and - in the aliases in combination with polyFunctions. This example does not work (for me): [Gating Template with + and -][1] alias CD4+IL2+ CD4+IL2- ...
written 28 days ago by k.teijgeler0
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Comment: C: polyFunctions gate in OpenCyto 1.20
... Thanks for looking at it, somehow that Gating Template works for me the boolGate is not giving me a problem The following does not work for me: [polyFunctions Test][1] [1]: https://uploadfiles.io/n2q6kwg1 I installed R 3.6 and just tried the Bioc release 3.9 but i am getting the same error: [180 ...
written 29 days ago by k.teijgeler0
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Comment: C: polyFunctions gate in OpenCyto 1.20
... Its weird, the problem starts arising when i add the last line, the 2 tmix gates worked fine for me. To check if those 2 lines are causing the problem i've made the following Gating Template: [polyFunctions test Gating Template][1] But i am getting a similar error: [125] "Population '*'" ...
written 29 days ago by k.teijgeler0
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Comment: C: polyFunctions gate in OpenCyto 1.20
... here is the CSV file: [Gating Template CSV][1] [1]: https://ufile.io/o5l83law Thanks for looking at it ! ...
written 29 days ago by k.teijgeler0
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polyFunctions gate in OpenCyto 1.20
... Hello, I am trying to make a csv GatingTemplate using OpenCyto 1.20.2. All gating functions i've used thusfar have worked. The current panel im working on has a lot of polyFunctionality so i would love to use the polyFunctions gate for this. In the previous csv GatingTemplate style i remember i ha ...
opencyto polyfunctions polyfunctionsgate written 29 days ago by k.teijgeler0 • updated 24 days ago by Jiang, Mike1.2k
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Comment: C: RefGate populations switched in OpenCyto 1.20
... Thanks a lot Mike for the quick solution! :) ...
written 4 months ago by k.teijgeler0

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