User: rogangrant

rogangrant • 0

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Posts by rogangrant

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Comment: C: Perfectly aligning sequences not mapping to worm genome with Subread-align?

... I re-ran the alignment with STAR yesterday, and the output confirms that multi-mapping is the issue, thank you! Still left to wonder why multi-mapping is higher in data from stress conditions, but that's probably more of a biological question. Now I just need to find a way to rescue some of these mu ...

written 11 months ago by rogangrant • 0

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Perfectly aligning sequences not mapping to worm genome with Subread-align?

... I am currently trying to optimize RNAseq analysis for stress conditions, in which splicing dysregulation (among other issues) is a major obstacle to accurate mapping. I therefore thought to try genome/split-read alignment over direct transcriptome mapping. Using the subread package with existing hea ...

rnaseq featurecounts subread written 11 months ago by rogangrant • 0 • updated 11 months ago by Gordon Smyth ♦ 37k

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