User: Matthew.baldwin

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Posts by Matthew.baldwin

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Comment: C: Deseq 2 multiple comparisons (3 groups, 24 samples)
... Thanks Michael.  I have thought further and noted in the vignette about grouping interactions. I have refined my 3 questions: 1) For scaffolds with aligned fibre orientation, what is the effect of fibre diameter ?( i.e. 300Aligned vs 1000Aligned, 300Aligned vs 2000Aligned and 300Aligned vs 4000Ali ...
written 13 months ago by Matthew.baldwin0
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Comment: C: Deseq 2 multiple comparisons (3 groups, 24 samples)
... Hi Michael,  It would be fine to model as a diameter as a numeric e.g. 0.3, 1,2,4. We believe that the greatest difference will be for the smallest i.e. 0.3um and largest diameters i.e. 4um ...
written 14 months ago by Matthew.baldwin0
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Comment: C: Deseq 2 multiple comparisons (3 groups, 24 samples)
...   Thank Michael,  Grateful for your help. The goal for the analysis are several fold: 1) Which factor (anisotrophy or diameter) produces the greatest change at an RNA level for tendon cells 2) Which genes are influenced by different anisotrophy (random vs aligned) 3) Which genes are influences ...
written 14 months ago by Matthew.baldwin0
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Deseq 2 multiple comparisons (3 groups, 24 samples)
... Dear all,  I am hoping you can help the correct test for my experimental design which is as follows: I tested the cells of 3 different donors (rotator cuff tears), on 8 different nano fibre scaffolds (different anisotropy; random vs aligned and different fibre diameters; 300nm,1000nm,2000 and 4000) ...
deseq written 14 months ago by Matthew.baldwin0 • updated 14 months ago by Michael Love26k

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