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Posts by hamza_karakurt
... Hey Aaron, Thank you for your answer. I will use calcNormFactors in scater. After this line, I need to multiply my SumFactors with my counts to normalize right? Or after computeSumFactors, directly normalize(sce) command does not do the job? I am working on unique barcoded single cell RNA-Seq. ...
... Hello everyone, I am trying to replicate a single cell RNA-Seq data analysis. Actually, the main problem is version. The old results were generated via newSCESet and normalized with normaliseExprs command (TMM method) but for the newest version of R, Bioconductor and packages so I used SingleCellEx ...
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