User: phickner2

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phickner20
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Posts by phickner2

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Comment: C: CalcNormFactors in Edge r
... I don't know how to go from a de novo transcriptome assembly that has over 400,000 contigs to a transcriptome that contains ~18,000 transcripts in its entirety. In my opinion, mapping back to the Trinity output will not produce reliable data. Furthermore, how do I generate a gff for an unknown trans ...
written 4 weeks ago by phickner20
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CalcNormFactors in Edge r
... Can anyone explain the "CalcNormFactors" normalization process in edge r?  I have RNAseq data from insect antennae. I do not have a genome assembly for my organism, but I did assemble the transcriptome using trinity. I then retrieved my transcripts of interest (chemosensory genes) from the de novo a ...
edger written 4 weeks ago by phickner20 • updated 4 weeks ago by Gordon Smyth36k

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