User: carlos.x.martinez-fleites

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Posts by carlos.x.martinez-fleites

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Comment: C: Flow cytometry optimization of transformation parameters
... Hi SamGG and Mikhael, thank you very much for your replies, I agree that pooling all data to estimate parameters seems a better idea that selecting a single frame, i will implement this suggestion. I will also try to implement looping through channels for the arcsinh estimation if there isn't an i ...
written 3 days ago by carlos.x.martinez-fleites0
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Flow cytometry optimization of transformation parameters
... Hello, I would like to optimise the parameters for an arcsinh transformation of flow cytometry data. I can optimise logicle parameters **per channel** as: *best_parameters <- estimateLogicle(frame, channels = channels, type = "data")* and then apply transformation as: *best_transformation < ...
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Comment: C: flowCore: retain raw data in the flowset after transformation
... Thanks, this works nicely. I want to keep all columns in the flowset for subsequent conversion into a single data.frame that I use as a data source for ggplot. ...
written 9 weeks ago by carlos.x.martinez-fleites0
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flowCore: retain raw data in the flowset after transformation
... Hello, Using flowcore functions, I would like to keep columns containing raw data in a flowset after applying a transformation, example: > logicleT <- logicleTransform( w = 0.5, t= 10000, m =4.5) > transformation <- transformList(c("FL2-A", "FL3-A", "FL6-A", "FL10-A"), logicleT ) > ...
flowcore flow cytometry written 9 weeks ago by carlos.x.martinez-fleites0 • updated 9 weeks ago by Jiang, Mike1.2k

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