User: galen.seilis

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Appropriate design formula for DESeq2 from principles
... **Background** I am analyzing RNA-seq reads to look at transcriptomic response to environmental stress, and I want to ensure that I am using the correct design formula for my experimental design. I have a condition factor with a control level and four other levels in no particular order (CL, A, B, ...
deseq2 written 5 weeks ago by galen.seilis0 • updated 5 weeks ago by Michael Love22k
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Comment: C: Designing a contrast in DESEQ
... Why should variables of interest go at the end of design formulae? I've briefly read about sequential sums of squares. Are sequential sums of squares calculated in DESeq2, and (if so) are they calculated in the order that terms appear in the design formula? ...
written 7 weeks ago by galen.seilis0

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