User: leekun4

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leekun40
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Posts by leekun4

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Comment: C: Getting opposite log2 fold change
... Hi Michael, I wasn't sure what you meant by that (I'm new to coding). I found out that it's doing uninfected vs infected instead of infected vs uninfected. res <-results(dds, contrast = list("infected", "uninfected")) I tried this to change but i'm getting an error. Any suggestions? Than ...
written 4 months ago by leekun40
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Comment: C: Getting opposite log2 fold change
... I really appreciate your help. When I typed res, I got this message: log2 fold change (MLE): dex uninfected vs infected Wald test p-value: dex uninfected vs infected DataFrame with 6 rows and 6 columns Any suggestions? ...
written 4 months ago by leekun40
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Getting opposite log2 fold change
... I am currently doing DESeq2 analysis, and I am getting opposite log2 fold change when I plot on Volcano plot. For example, one of my genes is down-regulated (with significant p-value) according to DESeq2; however, it says otherwise when I look at the actual gene expression data. What am I doing w ...
deseq2 R gene expression log2foldchange written 4 months ago by leekun40 • updated 4 months ago by Michael Love24k

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