User: bio.erikson

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Posts by bio.erikson

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RUVseq: Use of known controls with RUVg
... I have a dataset analyzing global transcription shifts. And the samples have been spiked with a known but unequal amount of ERCC; Such that each sample has constant ratio of biological RNA to Spike-in . It states in the RUVseq vignette: > Note that one can relax the negative control gene assump ...
ruvseq written 5 months ago by bio.erikson0
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EdgeR: Filtering Counts Causes No Significance.
... When I filter my count data with the code in the user guide, the FDR for all my genes drops to 1.0. But, if I don't filter or set the CPM cut off to ~0.2, then I start to get significant DE genes. I'm a bit confused by this behavior. What might be causing it? My experiment is in Xenopus Laevis, whe ...
edger written 5 months ago by bio.erikson0 • updated 5 months ago by Gordon Smyth38k

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