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Posts by bio.erikson
... I have a dataset analyzing global transcription shifts. And the samples have been spiked with a known but unequal amount of ERCC; Such that each sample has constant ratio of biological RNA to Spike-in . It states in the RUVseq vignette: > Note that one can relax the negative control gene assump ...
... When I filter my count data with the code in the user guide, the FDR for all my genes drops to 1.0. But, if I don't filter or set the CPM cut off to ~0.2, then I start to get significant DE genes. I'm a bit confused by this behavior. What might be causing it? My experiment is in Xenopus Laevis, whe ...
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