User: sidreed34

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sidreed340
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Posts by sidreed34

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Comment: C: scale_counts targetSize normalization for recount2 counts
... Thank you very much, this helps clarify a lot for me. For question 2 a better way to phrase it would have been "Why specifically would running scale_counts() and read_counts() return nonsensical results?" ...
written 5 days ago by sidreed340
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Comment: C: scale_counts targetSize normalization for recount2 counts
... Hello, Thank you for your response, however I am still confused about some things. 1. When you say scaling to the same AUC, do you mean dividing each sample column by it's AUC value, to account for coverage differences between samples? 2. Is accounting for coverage *and* mapped reads redundant ( ...
written 6 days ago by sidreed340
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scale_counts targetSize normalization for recount2 counts
... I am currently trying to conduct a differential expression analysis using the recount data. I want to compare the GTEx and TCGA data against a specific set of separate runs I have picked out. My issue is with the targetSize argument in the scale_counts function. By default it is set 4e7 for all run ...
recount written 14 days ago by sidreed340 • updated 7 days ago by Leonardo Collado Torres640

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