User: msubramanian1

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Posts by msubramanian1

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Comment: C: Low pseudo-alignment rate with Kallisto
... Oh thank you so much for clarifying! Appreciate all the help I can get as I work through all this :) ...
written 18 days ago by msubramanian10
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(Closed) Low pseudo-alignment rate with Kallisto
... Hi, I am new to bioinformatics and am trying to perform differential expression analyses on some mouse RNA-seq data. We performed Tru-Seq Strand Specific Large Insert RNA Sequencing - High Coverage (50M pairs) on the sample. I am now trying to pseudo-align the reads to the mouse transcriptome usin ...
kallisto written 18 days ago by msubramanian10
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Help with formula design for DESeq2
... Hi, I am new to bioinformatics and am running the DESeq2 pipeline on a 8 murine RNA-seq samples. I am having some trouble understanding how best to set up my design formula. The basic structure of my experiment is as follows: I have 8 samples: Samples 1 and 2 (biological replicates) : Cell line A; ...
deseq2 written 28 days ago by msubramanian10 • updated 25 days ago by Michael Love24k

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