User: aapapaiwannou

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Posts by aapapaiwannou

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Comment: C: Problem with DESeq2 object
... # Import data from featureCounts countdata <- read.table("genecounts.txt", header=TRUE, row.names=1) # Remove first five columns (chr, start, end, strand, length) countdata <- countdata[ ,6:ncol(countdata)] # Remove .bam or .sam from filenames and the firstpart "..mapping" colnames(countdat ...
written 26 days ago by aapapaiwannou0
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Problem with DESeq2 object
... Hello, I usisn DESeq2 package in R for analysing Rna seq sngle end data. After running the DESeq pipeline dds <- DESeq(dds) I am getting this warning message : In class(object) <- "environment" : Setting class(x) to "environment" sets attribute to NULL; result will no longer be an S4 objec ...
deseq2 biocodunctor written 27 days ago by aapapaiwannou0 • updated 26 days ago by Michael Love26k
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Comment: C: Calculating FPKM from raw counts data using fpkm() in deseq2
... Hello, I am having the same question. So I have a matrix with the gene length which i calculated from the gff file and I want to add this in the dds in order to run the fpkm(dds) can you help me with this? ...
written 4 weeks ago by aapapaiwannou0

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