User: Leonardo Rocha
Leonardo Rocha • 40
- Reputation:
- 40
- Status:
- New User
- Location:
- Last seen:
- 12 years ago
- Joined:
- 12 years, 5 months ago
- Email:
- l****************@gmail.com
Posts by Leonardo Rocha
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... Hi Pingzhao,
The read.imagene command couldn't find the column assigned for
background,
once in your file its named "Background Mean", while the default is
"Signal
Median, or Signal Mean if auto segmentation has been used" (take a
look at
Limma User's Guide page 14), so you must set the columns for ...
written 12.0 years ago by
Leonardo Rocha • 40
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... Hello Pingzhao,
Try the following commands:
library (limma)
targets <- readTargets ("targets.txt"); targets
targets1 <- as.matrix(read.table("targets.txt", header = TRUE));
targets1
targets2 <- matrix (c(targets1[,3], targets1[,2]),nrow=16,ncol=2);
targets2
RG <- read.imagene(targ ...
written 12.0 years ago by
Leonardo Rocha • 40
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Answer:
A: Limma and Imagene flags
... Hello Sally,
You should try the following commands:
myfun <- function(x) as.numeric(x$Flag ==0)
RG <- read.imagene(filenames$target, wt.fun=myfun)
I hope it helps.
Good luck.
Leonardo
----- Original Message -----
From: "Sally"
To:
Sent: Tuesday, November 06, 2007 9:36 PM
Subject: Re: ...
written 12.1 years ago by
Leonardo Rocha • 40
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... Dear List,
I am very sorry for the previous emails, I do not know what happened,
so I
trying to use another email. I am looking for help to account for
duplication in analysis using lmFit in limma of data from a two-
channel
microarray. The experiment is comparing differences between breeds (A
and ...
written 12.5 years ago by
Leonardo Rocha • 40
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