User: Leonardo Rocha

Reputation:
40
Status:
New User
Location:
Last seen:
12 years ago
Joined:
12 years, 5 months ago
Email:
l****************@gmail.com

Posts by Leonardo Rocha

<prev • 4 results • page 1 of 1 • next >
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Answer: A: RES: LIMMA: load two-color data (Imagene format)
... Hi Pingzhao, The read.imagene command couldn't find the column assigned for background, once in your file its named "Background Mean", while the default is "Signal Median, or Signal Mean if auto segmentation has been used" (take a look at Limma User's Guide page 14), so you must set the columns for ...
written 12.0 years ago by Leonardo Rocha40
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Answer: A: LIMMA: load two-color data (Imagene format)
... Hello Pingzhao, Try the following commands: library (limma) targets <- readTargets ("targets.txt"); targets targets1 <- as.matrix(read.table("targets.txt", header = TRUE)); targets1 targets2 <- matrix (c(targets1[,3], targets1[,2]),nrow=16,ncol=2); targets2 RG <- read.imagene(targ ...
written 12.0 years ago by Leonardo Rocha40
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Answer: A: Limma and Imagene flags
... Hello Sally, You should try the following commands: myfun <- function(x) as.numeric(x$Flag ==0) RG <- read.imagene(filenames$target, wt.fun=myfun) I hope it helps. Good luck. Leonardo ----- Original Message ----- From: "Sally" To: Sent: Tuesday, November 06, 2007 9:36 PM Subject: Re: ...
written 12.1 years ago by Leonardo Rocha40
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duplicated spots on oligonucleotide array
... Dear List, I am very sorry for the previous emails, I do not know what happened, so I trying to use another email. I am looking for help to account for duplication in analysis using lmFit in limma of data from a two- channel microarray. The experiment is comparing differences between breeds (A and ...
limma written 12.5 years ago by Leonardo Rocha40

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