User: Aric Gregson

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Aric Gregson270
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Posts by Aric Gregson

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Plot Multiple 'Backgates' Simultaneously flowViz
... Hello, I'm wondering if there is a way to demonstrate multiple back-gated populations within the same xyplot with smooth=F. For example, we create a gate based upon a previously defined quadrantGate: > rect.eff.cd8.gate <- rectangleGate("PerCP.Cy5.5.A"=c(0, + quadGate.memory.CD8 at boundary ...
written 7.7 years ago by Aric Gregson270
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Answer: A: Adding Multiple quadrantGates of Same Channels to workFlow (flowCore)
... On Mon, 14 Nov 2011 15:58:09 -0800 Aric Gregson wrote: > Is there a way around this? OK. I have come up with a way around this, although considering a more global answer for working with the workFlow and perhaps a different naming scheme for nodes/gateViews may be better in the long run. I ca ...
written 7.8 years ago by Aric Gregson270
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Adding Multiple quadrantGates of Same Channels to workFlow (flowCore)
... Hello, What I would like to do seems like a common application in analysis and I am probably missing a very easy way to accomplish this. I would like to apply a 'memory gate' generated by quadrantGate to both CD4 and CD8 lymphocyte populations. However, there appears to be no way to give the second ...
written 7.8 years ago by Aric Gregson270
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Sudden Issue with add for lymphGate in flowCore
... Hello, I have an odd issue that I have been unable thus far to resolve. Literally overnight it has become impossible to add a lymphGate filter to a workFlow. This is specific for the lymphGate filter only, as any other type of filter works just fine. The error that I am receiving is shown after the ...
written 7.8 years ago by Aric Gregson270 • updated 7.8 years ago by Martin Morgan ♦♦ 23k
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Comment: C: flowStats quadrantGate and 'names'
... On Thu, 23 Sep 2010 14:48:38 -0700 Nishant Gopalakrishnan wrote: > I am not sure what 'names' function in flowViz you are talking about. > I do not see one in flowViz. Its hard to guess what you have done > without providing some reproducible lines of code. > > With regard to puttin ...
written 8.9 years ago by Aric Gregson270
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flowStats quadrantGate and 'names'
... Hello, I have used the 'names' function in flowViz to place percents of the gated population within the plot. I have never figured out how to get the stats placed in the plot automatically, but at least I could place the numbers in the plot. With quadrantGate plots the 'names' function does not app ...
flowviz written 8.9 years ago by Aric Gregson270 • updated 8.9 years ago by Nishant Gopalakrishnan340
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Answer: A: Odd Plotting Behavior with lymphGate flowStats
... I believe this is just a plotting problem. The positive population in the workFlow appears to be the correct one, despite the median values. Carrying-on through many iterations of the plots confirms this. thanks, Aric ...
written 9.1 years ago by Aric Gregson270
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Odd Plotting Behavior with lymphGate flowStats
... There may be a problem with the lymphGate plot function and potentially the entire function. While generating the lymphGate, the plot=TRUE option shows the correct population is selected. However, when using the non-Data() plotting technique after to show the populations (as demonstrated in '...A ty ...
written 9.1 years ago by Aric Gregson270
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flowStats Plot quadrantGate Filter Result Smooth=FALSE
... Hello, I don't believe that I have seen an explanation and I suspect that I am not calling the filter appropriately. Using flowStats in a workFlow with a quadrantGate making a filter I can easily plot the results with the xyplot if smooth=TRUE (the default). However, when the populations get small ...
flowstats written 9.1 years ago by Aric Gregson270
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flowStats quadrantGate and rangeGate Question
... Hello, I am trying out the quadrant and rangeGate methods in flowStats with a small sample of data. It seems that they do not play well with asinh transformed data when there is a small, but significant, population below zero. I have tuned the adjustable parameters sd, alpha and borderQuant as much ...
flowstats written 9.2 years ago by Aric Gregson270 • updated 9.2 years ago by Chao-Jen Wong580

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