## User: Robert Baer

Robert Baer70
Reputation:
70
Status:
Trusted
Location:
United States
Last seen:
1 year, 3 months ago
Joined:
7 years, 8 months ago
Email:
r****@atsu.edu

#### Posts by Robert Baer

<prev • 19 results • page 1 of 2 • next >
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... I get the error in the title when I try to install the openCyto package or when I do a straight forward run of biocLite() with no arguments. In the case of openCyto I get the message at the end after an appaently successful install of openCyto.  Subsequently, "library(openCyto) fails ostensibly bec ...
written 16 months ago by Robert Baer70 • updated 11 months ago by Jiang, Mike1.2k
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... I've been working my way through the flow core demo codec and encountered a problem on line 157: > summary(filter(smaller[[1]],rectGate %&% morphGate)) Error in as.vector(data) :    no method for coercing this S4 class to a vector > summary(filter(smaller[[1]],rectGate & morphGate)) F ...
written 16 months ago by Robert Baer70 • updated 16 months ago by Jiang, Mike1.2k
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... In installing and updating  bioconductor packages the dependency GO.db fails on widows on what looks like a parsing error brought about by a space in the path on E: drive.  I don't know if this is because the linux equivalent is "/e/program files/..." to the Windows "E:\progem Files\..." But, wheth ...
written 22 months ago by Robert Baer70
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Comment: C: flowViz and grid
... Thanks as always for the quick feedback and suggestions. Thanks also for mentioning the future of flowViz --> ggcyto transition.  Somehow, I've missed ggcyto up until now.  I'm trying to put so much information together (as someone completely new to flow optometry) that these sorts of tips are e ...
written 2.5 years ago by Robert Baer70
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... When plotting flowFrames, I sometimes get the warning, "Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'. I have a general sense of what this is telling me in terms of the density plot, but I cannot figure out how to set the gridsize from the high order plotting ...
written 2.5 years ago by Robert Baer70 • updated 2.5 years ago by Jiang, Mike1.2k
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... # flowCore’ version 1.38.1 Steps to reproduce: 1. Read an .fcs file created with a BioRad S3 cell sorter using f <- read.FCS(.) # Names of  in flowFrame names(f@description)   [1] "FCSversion"        "$BEGINANALYSIS" "$ENDANALYSIS"        [4] "$BEGINDATA" "$ENDDATA"          "\$BEGIN ...
written 2.7 years ago by Robert Baer70 • updated 2.7 years ago by Jiang, Mike1.2k
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... Beautiful! I've now installed  flowCore Version: 1.35.12 and the original  BioRad S3 cell sorter file appears to read seamlessly.   Thanks for making your package so accessible.  We are grateful for your contributions.   Biorad tech support reports to me that they too are now able to read files wi ...
written 3.3 years ago by Robert Baer70
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... Thanks, again.  I have contacted ProSort support to see what I can get accomplished.  Your patience with my questions greatly appreciated.  I have referenced this discussion for their edification.     ...
written 3.3 years ago by Robert Baer70
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... Follow-up The ProSort software does not support float/double saving. I have investigated the headers of these ProSort FCS files, and can provide more detail on what I think is happening. flowCore and read.FCS() are apparently not having difficulty reading data, but rather are having difficulty rea ...
written 3.3 years ago by Robert Baer70
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... Thanks.  That is at least a start toward a possible resolution.   I don't know yet what the save options are on the Biorad (ProSort) software, but I'll check it out .  You seem to be telling me that the solution lies on the export side, not the import side.  This implies that the fcs format saves v ...
written 3.3 years ago by Robert Baer70

#### Latest awards to Robert Baer

Popular Question 22 months ago, created a question with more than 1,000 views. For Warning messages with biocLite()

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