User: sbcn

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sbcn50
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Posts by sbcn

<prev • 17 results • page 1 of 2 • next >
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Comment: C: GenomicFeatures::makeTxDbFromEnsembl fails to create a TxDb object
... For information, I got the same error using makeTxDbFromEnsembl but makeTxDbFromBiomart works properly (here I'm getting the release 89 / May 2017 for Mus musculus): TxDb_EnsRel89_Mm <- makeTxDbFromBiomart(biomart="ENSEMBL_MART_ENSEMBL", dataset="mmusculus_gene_ensembl", host="may2017.archive.en ...
written 13 days ago by sbcn50
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RiboProfiling: offset graph / shiftValue
... Hi, I am analysing my first Ribo-seq project and I am giving the "RiboProfiling" package a try. I produced the "offset graphs" (Offset around TSS for the best expressed CDSs on a subset of match sizes) for one sample. In the vignette - if my understanding is correct - the offsets calculated for v ...
riboprofiling ribo-seq written 16 days ago by sbcn50
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Comment: C: HTqPCR does not copy featureData when subsetting a qPCRset
... Hi Henrik, I wanted to include your function modification but, in the corresponding folder in my R library (mylib/HTqPCR/R), I do not find any R file corresponding to the qPCRset function. I've looked around in other folders but I don't find it... Where do you include this code? Does it belong to ...
written 6 weeks ago by sbcn50
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Comment: C: DESeq2 on CIRI results ?
... Ok I see... Then I'll try and assess the variability in expression across all samples, and see then how I proceed. Thanks for the support! ...
written 10 weeks ago by sbcn50
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Comment: A: DESeq2 on CIRI results ?
... Hi Michael, Thanks for your reply! I have counts for around 1000 circRNAs: do you think that is enough? Thanks again, Sarah   ...
written 10 weeks ago by sbcn50
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DESeq2 on CIRI results ?
... Hi, I am analysing a RNA-seq data set of 6 samples (2 conditions in biological triplicates); the goal is to identify the differential expressed circular RNAs in one condition versus the other. I used the CIRI2 program (v2.0.6) to detect circRNAs from the RNA-seq data. As an output, I get the circ ...
deseq2 circrna ciri ciri2 written 10 weeks ago by sbcn50
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Comment: C: limma regularization on Ballgown
... Hi Alyssa, I am a bit confused how to go from stringtie estimated expression per transcript to limma via ballgown (I am working with triplicates and trying to do some differential expression analysis at the transcript level; so, as the ballgown documentation advises, I will use limma). In the limm ...
written 10 months ago by sbcn50
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Comment: C: DESeq2: interaction term as in limma
... Thank you Simon for the clarification. Then the interaction is indeed what I am looking for. Best,   ...
written 14 months ago by sbcn50
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Comment: C: DESeq2: interaction term as in limma
... Thanks a lot Simon for your prompt answer! It is a lot more clear now to me what each term means. If a gene is differentially expressed in B_ko - B_ctrl but not in A_ko - A_ctrl (change in expression triggered by the KO in cell line B but not in cell line A), it will come out of the interaction an ...
written 14 months ago by sbcn50
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DESeq2: interaction term as in limma
... Hi, In limma's documentation, the interaction term is described as: "The first of these questions relates to the WT.S vs WT.U comparison and the second to Mu.S vs Mu.U . The third relates to the difference of differences, i.e., (Mu.S-Mu.U)-(WT.S-WT.U) , which is called the interaction term." I wan ...
limma deseq deseq2 interaction term written 14 months ago by sbcn50 • updated 14 months ago by Simon Anders3.5k

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Popular Question 14 months ago, created a question with more than 1,000 views. For ballgown custom models

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