User: Fletez-Brant, Christopher NIH/VRC [C]

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Posts by Fletez-Brant, Christopher NIH/VRC [C]

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FW: Using HTqPCR for Fluidigm BioMark input data (48.48 array)
... Just following my own advice here. On 11/19/12 9:22 AM, "Fletez-Brant, Christopher (NIH/VRC) [C]" wrote: >Hi Jens, > >Sorry for taking so long to get back to you - I was out of town. A couple >of things: > >1) Please always use 'reply to all', as we use the Bioconductor mailing ...
clustering htqpcr written 6.8 years ago by Fletez-Brant, Christopher NIH/VRC [C]60
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Answer: A: Using HTqPCR for Fluidigm BioMark input data (48.48 array)
... Jens, If your qPCRset recognizes that there are 47 features and 3 samples, then you should be able to perform downstream analyses. If you are having trouble modifying sample names, I have been storing sample names in a file, ordered as the actual samples are in the Fluidigm output file, then assig ...
written 6.8 years ago by Fletez-Brant, Christopher NIH/VRC [C]60
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Comment: C: flowQ loading issue
... Thank you for the reply. I reinstalled ImageMagick per their instructions (that is, as I am using a Mac I installed via MacPorts), and when I run the convert command I get the attached output. However, R gives me the same error. Any help is appreciated. Thank you, Kipper convert -list configur ...
written 7.1 years ago by Fletez-Brant, Christopher NIH/VRC [C]60
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flowQ loading issue
... Dear List, I am attempting to load flowQ and am getting the error message listed at the end of this message. It is similar to errors reported here: https://stat.ethz.ch/pipermail/bioconductor/2011-June/039962.html and here: https://stat.ethz.ch/pipermail/bioconductor/2011-August/040806.html Ho ...
convert flowq written 7.1 years ago by Fletez-Brant, Christopher NIH/VRC [C]60 • updated 7.1 years ago by Greg Finak240
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Comment: C: HTqPCR - limmaCtData using samples with different number of replicates?
... Hi Heidi, Thanks for setting me straight - I in fact have a panel of 96 unrepeated features, used to produce experiments with a variable number of replicates per group. This works very well. Thanks! On 6/26/12 12:14 PM, "Heidi Dvinge" wrote: >Hi Kipper, > >> Dear List, >> > ...
written 7.2 years ago by Fletez-Brant, Christopher NIH/VRC [C]60
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HTqPCR - limmaCtData using samples with different number of replicates?
... Dear List, I am using HTqPCR to analyze Fluidigm data (specifically, the 96*96 format). I have been using specifically limmaCtData to look for differential expression between groups, always having the same number of replicates. However, I currently have a dataset of several groups with 3 replicat ...
htqpcr written 7.2 years ago by Fletez-Brant, Christopher NIH/VRC [C]60 • updated 7.2 years ago by Heidi Dvinge2.0k

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Popular Question 6.8 years ago, created a question with more than 1,000 views. For FW: Using HTqPCR for Fluidigm BioMark input data (48.48 array)

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