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... ​​Dear Pariksheet Thanks again for reporting. I have not been able to reproduce the issue on our local environment (CentOS Linux release 7.6.1810 (Core), gcc 9.1.0, binutils 2.32, zlib 1.2.11, R 3.6.0, BioC 3.9). Compile flags seem to be mostly default and the same as yours (especially CXX, which ...
written 11 weeks ago by Michael Stadler330
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... Dear Pariksheet Thanks for the report. I will try to reproduce this on our local environment and let you know when I find a fix. Best, Michael On 25 May 2019, at 16:18, Pariksheet Nanda [bioc] <noreply@bioconductor.org<a rel="nofollow" href="mailto:noreply@bioconductor.org">noreply@bioco ...
written 11 weeks ago by Michael Stadler330
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... Hi Vakul You should probably rather use a GRanges query, instead of a GRangesList. The GRangesList query is meant for a special analysis (see ?qCount) which partitions the genome into domains. If you want one count per exon, use a GRanges with exons without names or with unique names pe ...
written 5 months ago by Michael Stadler330
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... Hi Dario The documentation and also plotting issues have been addressed in QuasR 1.23.17 (will become available in BioC-devel in a day or so). The documentation has been simplified to make it clear that some plots will not be available if the necessary information is missing (e.g. base quality plo ...
written 5 months ago by Michael Stadler330
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... Dear Rahel, Regarding splicedAlignment=TRUE: As you are using external bam files, this parameter will not have any effect on the alignments themselves. It would only matter if you are doing the alignments within QuasR. It's purpose is here to "tell" QuasR that the bam files may contain spliced alig ...
written 4.0 years ago by Michael Stadler330
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... Dear Rahel, My guess is that you have a problem in the file "sample_file.txt". Make sure that it only contains two columns in each row, separated by a single tab-character. The first error message that you got ("... should only contain the columns names ...") is only thrown when your samples file c ...
written 4.0 years ago by Michael Stadler330
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... Dear Dani, Please have a look at the documentation of qAlign. The sampleFile argument takes the path and filename of a text file, which in turn contains the paths and file names of the sequence or bam files. What you do is to directly pass the bam file name, which is not the same. QuasR comes wit ...
written 5.0 years ago by Michael Stadler330
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... Dear Dani, I hope you don't mind me cc'ing the bioconductor list. The question may be of interest to others. The answer to your questions is described in the vignette (section 5.1 "Create a sample file"). Essentially, you just list the pre-existing bam files instead of the sequence files. QuasR wi ...
written 5.0 years ago by Michael Stadler330 • updated 5.0 years ago by Daniel Soronellas10
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Comment: C: Rbowtie - compilation error
... Thanks Martin for notifying! Michael On 04.06.2014 13:22, Martin Morgan wrote: > Please remember to include the package maintainer in mail about > individual packages. The maintainer is available from the package > 'landing page' > > http://bioconductor.org/packages/release/bioc/ht ...
written 5.2 years ago by Michael Stadler330
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... Hi Romain, Great, thank you very much for testing. I am copying the list again so others who might run into the problem are aware that an update to Rbowtie version 1.4.5 (release) or 1.5.5 (development) should solve the issue. Best wishes, Michael On 05.06.2014 18:06, Romain Retureau wrote: > ...
written 5.2 years ago by Michael Stadler330

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