User: guojiegina

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Posts by guojiegina

<prev • 4 results • page 1 of 1 • next >
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Answer: A: how to use negative controls to obtain scale and offset parameters then to norma
... Thanks again for your great patience. I did as you suggested. Please check it out as below. > m1 <- justvsn(min) vsn2: 96 x 149 matrix (1 stratum). Please use 'meanSdPlot' to verify the fit. > m2 <- justvsn(min[ rownames(min) != "mean-Rv-Negative", ]) vsn2: 95 x 149 matrix (1 stratum) ...
written 4.2 years ago by guojiegina0
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Answer: A: how to use negative controls to obtain scale and offset parameters then to norma
... so you recommend I treated the "mean-Rv-Negative" as another protein, say my dataset includes 96 kinds of protein(in fact 95 kinds of proteins expressed from 85 ORFs plus one "no DNA" control) and use justvsn method to transform the all 96 proteins data instead of leave the "no DNA" control out? I'm ...
written 4.2 years ago by guojiegina0
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Answer: A: how to use negative controls to obtain scale and offset parameters then to norma
... Dear Dr. Huber, Thank you for your reply. I thought Chapter 7 was all about positive controls or features which were differentially expressed.Now I get it that it can also apply to negative controls. But the example in the chapter says that taking features 100 to 200 as spike-in controls then to n ...
written 4.2 years ago by guojiegina0
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how to use negative controls to obtain scale and offset parameters then to normalize the whole protein microarray data by vsn method
... Hello everyone, Recently I have been analyzing my protein microarray data. I choose vsn method to process the raw data as many relative researches did. My data looks like as below: protein name T1 ~ T87 N1 ~ N62 ...

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