User: serpalma.v

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serpalma.v30
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Germany
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3 months, 3 weeks ago
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Posts by serpalma.v

<prev • 32 results • page 1 of 4 • next >
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Meaning of the intercept when betaPrior is TRUE?
... If I run DESeq(betaPrior=FALSE) I get two coefficients: the intercept (group A) and the difference between condition B versus A. dds <- makeExampleDESeqDataSet() res1 <- DESeq(dds, betaPrior = FALSE) resultsNames(res1) [1] "Intercept" "condition_B_vs_A" If I run DESeq(betaPrior=TRU ...
deseq2 written 3 months ago by serpalma.v30 • updated 3 months ago by Michael Love18k
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Comment: C: Significantly regulated genes produced by only a few affected samples
... I plotted the shrinked fold changes against the mean and the extreme gene is now shown significant but almost not-regulated (please see figure in link). Sorry but I did not get the part of subsetting after lfc shrinking by abs(log2FoldChange) > 0.5. Did you mean to remove the subset having large ...
written 4 months ago by serpalma.v30
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Comment: C: Significantly regulated genes produced by only a few affected samples
... Hi Michael, the version is DESeq2_1.18.1 I did the changes you mentioned and LFC shrinkage worked. Thanks again!   ...
written 4 months ago by serpalma.v30
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Comment: C: Significantly regulated genes produced by only a few affected samples
... Trying to run lfcShrink for the coefficient cond2 vs cond1, using lfcShrink(dds2, coef=4) (first 3 coefficients are for the countries) gives "Error: subscript contains invalid names". I tried changing the coef variable to the name of the coefficient, and also using the contrast argument with both, t ...
written 4 months ago by serpalma.v30
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Significantly regulated genes produced by only a few affected samples
... Hello I used DESeq2 to perform differential expression between three groups (condition1, condition2 and condition3). Samples also come from three different countries. PCA (using rlog transformed data) showed a similar pattern of clustering using country and condition (please see link) So I controll ...
deseq2 written 4 months ago by serpalma.v30 • updated 4 months ago by Michael Love18k
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Should I contrast 2 treatment groups using the control group as reference or directly against each other?
... Dear community, I am analyzing RNAseq data set with DESeq2. Samples are grouped in three groups: one control group (n=16) and two case groups (case 1 and 2, n=6 and n=8, respectively). I want to find differentially expressed genes of both case groups against the control group, but also against eac ...
deseq2 contrast glm model.matrix written 6 months ago by serpalma.v30 • updated 6 months ago by Michael Love18k
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Comment: C: Multiple test corrections for planed contrasts
... The response says: If you control the FDR at a given level for each of 5 contrasts separately, then you have automatically controlled the FDR at the same level for all 5 contrasts together. I think is contradictory to what is writen in the DESeq2 Vignette (text cited in the initial post).  ...
written 7 months ago by serpalma.v30 • updated 7 months ago by Michael Love18k
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Multiple test corrections for planed contrasts
... I have a data set where samples are grouped according to their health status (3 levels) and sampling timepoints (2 levels for each sample). This would be a simplified version of the data set: df <- data.frame(status = c( rep("healthy", 4), rep("condition1", 4), rep("condition2", 4) ), ...
deseq2 contrast multiple comparisons written 7 months ago by serpalma.v30 • updated 7 months ago by James W. MacDonald46k
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Comment: C: Imputation method selection (mass spectrometry) bimodal distribution
... Thank you very much Laurent. I will go for the second method, as it is the one I can better understand how it works ...
written 7 months ago by serpalma.v30
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Imputation method selection (mass spectrometry) bimodal distribution
... I am currently processing a mass spectrometry data set with the Bioconductor package “Differential Enrichment analysis of Proteomics data” (DEP). The data set consists of 8 samples divided into two conditions, 4 animals per group. I followed the steps in the vignette of said package and I am at th ...
impute massspectrometry missing value imputation dep written 7 months ago by serpalma.v30 • updated 7 months ago by Laurent Gatto980

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Popular Question 18 months ago, created a question with more than 1,000 views. For Model matrix for anova in limma
Popular Question 18 months ago, created a question with more than 1,000 views. For Does limma calculate pvalues using log2 fold changes or with linear values?
Scholar 2.5 years ago, created an answer that has been accepted. For A: Model matrix for anova in limma

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