User: galib36

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galib3610
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United Kingdom
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2 months, 3 weeks ago
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2 years ago
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Posts by galib36

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Regarding scRNA seq vs Bulk RNA seq
... The last para of Aaron Lun's modified reply answers the question. In Bulk population cells were dominating with some very high expressed genes causing all the reads to go for those genes. This would not be dominated in single-cells because some of the cells would have those highly expressed genes mo ...
written 12 weeks ago by galib3610
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Answer: A: Regarding scRNA seq vs Bulk RNA seq
... Are these lower expressed genes? It could be that as there are fixed number of reads that are shared across the mRNAs, the highly expressed genes are taking the majority share of the reads and so the lowly expressed genes are not getting any read.   ...
written 12 weeks ago by galib3610
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Comment: C: Why Diffbind function dba.peakset gives 1
... Asking the following question just for a bit of more clarification, I have two sets of single cells, each composed of 96 cells. When using bedtools multimap, I combine the peakset from all these 192 cells and then count the overlapping peaks to have the count for each cell. I was wondering, is this ...
written 19 months ago by galib3610
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Answer: A: Why Diffbind function dba.peakset gives 1
... Thank you for the Clarification ...
written 19 months ago by galib3610
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Why Diffbind function dba.peakset gives 1
... Hello Rory, I am using diffbind to extract the raw counts for my PEAKS. I used the function myData.RawCount <- dba.count(myData, score=DBA_SCORE_READS) myData.RawCount.value <- dba.peakset(myData.RawCount, bRetrieve=TRUE, writeFile="../RawReads.txt") I am using it for Single-cell ATAC seq ...
diffbind written 19 months ago by galib3610
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Monocle causes R session to crash
... I was trying to use monocle. I started with the monocle-vignette.R code. But as soon as I run the command newCellDataSet() my R session crashes. It happens in RStudio as well as in R sessions in command prompt. Could you please suggest how to solve it. ...
software error monocle written 2.0 years ago by galib3610 • updated 4 months ago by kevin.rue200

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