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Error in contrasts<-(tmp, value = contr.funs[1 + isOF[nn]]) : contrasts can be applied only to factors with 2 or more levels
software error
ballgown
rstudio
R
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5.1 years ago
fatimarasool135
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ballgown time course design and command confirmation
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6.1 years ago
yadongxue
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What is the exact formula for the calculation of Fold Change (FC), p-val and q-val in Ballgown stattest?
ballgown
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fold change
updated 6.2 years ago by
James W. MacDonald
66k • written 6.2 years ago by
ag1805x
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Answer: Normalisation using clinical traits in RNA seq using DESeq2
by
ATpoint
★ 4.2k
See the vignette, it's a FAQ: https://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#why-after-vst-are-there-st…
Comment: More regions in union when increasing DBA$config$mergeOverlap
by
Ian D.
▴ 70
Please could you comment on this @rory
Comment: Using featureCounts with SAF annotation file gives "Error: only GTF files contai
by
Wei Shi
★ 3.6k
Your bam file names should be stored in a vector and then provided to the `files` parameter.
Comment: Volcano plot of scRNA-seq data not "centered"
by
Michael Love
42k
Agree with @atpoint Depending on the "groups" in single cell, the question of DE can be not well defined without inserting additional inf…
Answer: Volcano plot of scRNA-seq data not "centered"
by
ATpoint
★ 4.2k
Sounds like a normalization problem, but I am not sure how to help with that. Indeed you can use control genes, for example use the top 20%…
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Answer: Not able to install any Bioconductor 3.18 package [unable to access index]
Answer: Documenting resources in an `ExperimentHub` package?
Answer: Not able to install any Bioconductor 3.18 package [unable to access index]
Seurat/DESeq2 - single cell RNA-seq differential expression
Seurat to SingleCellExperiment Converting error in MAC
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