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Gary
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@gary-7967
Last seen 6.7 years ago
Hi,
I use makeTranscriptDbFromGFF in GenomicFeatures to access a mandarin duck genome annotation (.gff file) provided by our cooperators. However, it showed that Error in makeTranscriptDbFromGFF("Mandarin.gff", format = "gff") : could not find function "makeTranscriptDbFromGFF". Please see the detail below. Could you help me? Many thanks.
R version 3.4.1 (2017-06-30) -- "Single Candle"
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> setwd("/Volumes/NCUE/20170811_Tang_MandarinDuck/20170809_ReferenceGenomeAnnotation")
> require(GenomicFeatures)
Loading required package: GenomicFeatures
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: ‘BiocGenerics’
The following objects are masked from ‘package:parallel’:
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from ‘package:stats’:
IQR, mad, sd, var, xtabs
The following objects are masked from ‘package:base’:
anyDuplicated, append, as.data.frame, cbind, colMeans,
colnames, colSums, do.call, duplicated, eval, evalq,
Filter, Find, get, grep, grepl, intersect, is.unsorted,
lapply, lengths, Map, mapply, match, mget, order, paste,
pmax, pmax.int, pmin, pmin.int, Position, rank, rbind,
Reduce, rowMeans, rownames, rowSums, sapply, setdiff,
sort, table, tapply, union, unique, unsplit, which,
which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: ‘S4Vectors’
The following object is masked from ‘package:base’:
expand.grid
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: AnnotationDbi
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages
'citation("pkgname")'.
> sessionInfo()
R version 3.4.1 (2017-06-30)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Sierra 10.12.6
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.4/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] GenomicFeatures_1.28.5 AnnotationDbi_1.38.2 Biobase_2.36.2 GenomicRanges_1.28.5 GenomeInfoDb_1.12.2
[6] IRanges_2.10.3 S4Vectors_0.14.4 BiocGenerics_0.22.0
loaded via a namespace (and not attached):
[1] Rcpp_0.12.12 XVector_0.16.0 GenomicAlignments_1.12.2 zlibbioc_1.22.0
[5] BiocParallel_1.10.1 bit_1.1-12 lattice_0.20-35 rlang_0.1.2
[9] blob_1.1.0 tools_3.4.1 grid_3.4.1 SummarizedExperiment_1.6.4
[13] DBI_0.7 matrixStats_0.52.2 bit64_0.9-7 digest_0.6.12
[17] tibble_1.3.4 Matrix_1.2-11 GenomeInfoDbData_0.99.0 rtracklayer_1.36.4
[21] bitops_1.0-6 RCurl_1.95-4.8 biomaRt_2.32.1 memoise_1.1.0
[25] RSQLite_2.0 DelayedArray_0.2.7 compiler_3.4.1 Rsamtools_1.28.0
[29] Biostrings_2.44.2 XML_3.98-1.9
> txdb <- makeTranscriptDbFromGFF("Mandarin.gff", format="gff")
Error in makeTranscriptDbFromGFF("Mandarin.gff", format = "gff") :
could not find function "makeTranscriptDbFromGFF"

You are right and thank you so much.