cummeRbund grouping by TSS / CDS / Isoforms

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corend • 0

@corend-14293

Last seen 7.1 years ago

I posted here a moment ago as it is a general bioinformatics question, but I will ask again here as it is about a bioconductor package cummeRbund.

I am working on RNA-seq data with cufflinks. I now use cummeRbund to analyze my results.

cummeRbund performs expression quantification between genes / isoforms / CDS or TSS. I understand the difference between gene and isoform expression quantification, but I don't get the difference between TSS, CDS and isoform.

What does it mean to have a gene with differentially expressed isoforms but no DE CDS ? I think I don't get what do they mean when they group by TSS or CDS.

So what does it mean to have DE TSS / CDS / isoforms ? What's the difference between them ?

I couldn't find anything clear enough for me in the cummeRbund vignettes or manual.