Hi all,

I want to know how to get a gene expression value for a condition with different replicates.

For example, I have condition M and N, each condition with two replicates M1, M2, N1, N2

I want to get one value to represent the gene expression value (FPKM or TPM) of M, can I just use the mean of each replicate? M=(M1+M2)/2?

Is there any other way to calculate the gene expression value for a condition?

Any advice is appreciated!

Thank you very much!!