"input object should be a named list" error in ChIPseeker
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k.panov • 0
@kpanov-15226
Last seen 3 months ago
United Kingdom

Hello,

I am analyzing two MACS2 bed files using ChIPseeker. Individual analysis is OK, but I'm getting an error message for simultaneous analysis of two files when using following ChIPSeeker functions: plotAnnoBar, plotDistToTSS and vennplot. All error messages are similar: Error in XXXX (peakAnnoList) :input object should be a named list (see example below). However, it all works for test files (provided with ChIPseeker) (see example below)

Any advice?

Best regards

Konstantin


> peakAnnoList <- lapply(files, annotatePeak, TxDb=txdb,

+                        tssRegion=c(-3000, 3000), verbose=FALSE)

> plotAnnoBar(peakAnnoList)

Error in plotAnnoBar(peakAnnoList) :

  input object should be a named list...

> plotDistToTSS(peakAnnoList)

Error in plotDistToTSS(peakAnnoList) :

  input object should be a named list...

> genes= lapply(peakAnnoList, function(i) as.data.frame(i)$geneId)

> vennplot(genes)

Error in vennplot(genes) : input object should be a named list...

> files <- getSampleFiles()
> peakAnnoList <- lapply(files, annotatePeak, TxDb=txdb,
+                        tssRegion=c(-3000, 3000), verbose=FALSE)
> plotAnnoBar(peakAnnoList)
> plotDistToTSS(peakAnnoList)
> genes= lapply(peakAnnoList, function(i) as.data.frame(i)$geneId)
> vennplot(genes)
ChIPseeker named list error plotAnnoBar plotDistToTSS vennplot • 2.4k views
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Entering edit mode
Guangchuang Yu ★ 1.2k
@guangchuang-yu-5419
Last seen 7 weeks ago
China/Guangzhou/Southern Medical Univer…

set the names of peakAnnoList, e.g.


names(peakAnnoList) <- basename(files)
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Cool, it works now! Thank you very much. Could I suggest to add this into ChIPseeker manual?

Best regards

Konstantin

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thanks for the recommendation.

 

In next release, it will throw warning msg (instead of error) that the input should be a named list and set the named automatically.

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