Hello,

I have human lung cancer cell data with no replicates, and following all the procedures in DESeq2.

After rlogTransformation， I got this table:

rld<-rlogTransformation(dds2),

My question is: is that possible I can calculate the foldchang of two samples just use this table?

Thank you in advance!

Yup, the log2 fold change is just the difference between rlog() or vst() samples.

Thank you so much for your great help!